Preparation of Enzymatically-gellable Collagen-Chitosan Hydrogels forLiver Tissue Engineering

Collagen-chitosan composite hydrogels are attracting increasing attention due to their great potentialapplications in biomaterials. However, conventional collagen-chitosan hydrogels degradates rapidlydue to the lack of strong cross-linkings in their networks. In the present work, chitosan was modifiedwith lysine and then used along with collagen in the preparation of composite hydrogels. Thecrosslinking enzyme microbial transglutaminase was used to create chemical cross-linkings in thecomposite hydrogels. The micro-structure of the prepared hydrogels were observed using FESEM.Also, liver cell growth conditions were investigated on the prepared hydrogels. The preparedcollagen-chitosan composite hydrogels showed no toxicity and acted as a suitable scaffold for livercells growth.

کلیدواژه ها:

Polymers in Medicine and Healthcare ، Hydrogel ، Collagen ، Chitosan