The difference in gene expression network based on gender in the recovery of knee cruciate ligament injuries during exercise

Anterior cruciate ligament (ACL) injuries are common and often debilitating, requiring extensive rehabilitation. The recovery process involves complex physiological, biomechanical, and biochemical factors. Gender differences in the incidence and recovery from ACL injuries are well-documented, with recent advances in molecular biology suggesting that gene expression networks play a crucial role in tissue repair and regeneration. This study aims to compare gene expression networks between genders to provide strategies for better-managing sports injuries. Gene expression data were sourced from the Gene Expression Omnibus (GEO) database, with samples divided into four groups based on gender and age. Differentially expressed genes (DEGs) were identified using GEO۲R, and biological interaction networks were constructed using the STRING (Search Tool for the Retrieval of Interacting Genes/Proteins). Network analysis was performed using Gephi, focusing on betweenness centrality and overall centrality. Two common genes, TRNA Aspartic Acid Methyltransferase ۱ (TRDMT۱) and NCF۱, were identified in both sexes but exhibited different centrality measures. NCF۱, associated with the production of superoxide anion and linked to chronic granulomatous disease, was expressed ten times higher in men than women. TRDMT۱, responsible for RNA methylation, was twice as highly expressed in men. The study underscores the importance of gender-specific molecular mechanisms in ACL injury recovery and highlights the need for personalized treatment strategies.

کلیدواژه ها:

Anterior cruciate ligament ، gene network ، Bioinformatics ، knee cruciate ligament injuries ، Exercise