Association of SSR Markers for primary branches in Brassica Juncea L.

The study utilized F۲:۵ lines derived from a cross between Kafiav N Zagora and Pusa Karishma cultivars to tag genomic regions controlling primary branches in Brassica juncea. One hundred and thirty F۲:۵ plants were used to characterize primary branch numbers, resulting in two pools of ۱۲ genotypes for high (HPB) and low (LPB) branches. The average number of primary branches for HPB and LPB were ۱۲.۱۶ and ۴.۵۰, respectively. A set of ۱۴۸ SSR (Simple Sequence Repeats) markers was used for parental polymorphism screening from which ۱۴ polymorphic SSRs were used for molecular characterization of HPB and LPB bulks, tagging genomic regions. The allelic data scored for ۱۴ polymorphic lines was tested using Student’s t-test analysis to understand relationships for primary branches with SSR markers and amplified alleles. Based on this, two B-genome markers (Ni۲-C۱۲ and Ni۲-A۱۱) were discovered to be strongly linked to the number of primary branches. Bioinformatic analysis located these two markers within a ۹ Mb region on chromosome B۵ of B. juncea. Utilising F۲:۵ lines of an inter-gene pool genetic cross, the current study was able to locate the loci regulating the number of primary branches on B. juncea's sub-genome chromosome B۵. Before proceeding with fine-mapping investigations to dissect the genomic region (between ۵۵.۹ and ۶۴.۹ Mb) of sub-genome chromosome B۵, it is imperative to emphasize the necessity of verifying these results across diverse genetic backgrounds.

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Keywords: Brassica juncea ، primary branch number ، BSA ، SSR