Efficient in Vitro Callus Induction, Regeneration and Shoot Multiplication Protocols for Stachys inflata subsp. caucasica (Stschegl.); A Rare Medicinal Plant

Stachys inflata subsp. caucasica (Stschegl.) is an important medicinal plant, containing highly valuable compounds such as verbascoside, pinene, limonene, and myrcene. In this study, we report efficient in vitro callus induction and shoot regeneration protocols for S. stchschegleevii from two different explant sources of leaf and internode. The highest callus induction rate was observed in internode explants using MS medium complemented with ۰.۳ mg/l TDZ and ۰.۷ mg/l ۲,۴-D. The resulting calli were embryogenic with the ability to grow and maintain their structure on MS medium containing ۱.۵ mg/l BAP at high efficiencies (۹۰%). The β-gulucoronidase (gus) gene was then successfully transferred to the internode explants by the particle gun method, demonstrating their potential to express foreign genes. In conclusion, here we report efficient protocols for callus induction and regeneration of S. inflata subsp. caucasica that can be used in germplasm maintenance, production of secondary metabolites in in vitro cultures, and molecular breeding through genetic engineering.

کلیدواژه ها:

Stachys schtschegleevii ، In vitro regeneration ، Micro-propagation ، Lamiaceae ، Shoot multiplication