Evaluation of the Anti-inflammatory and Immunomodulatory Properties of Jatropha multifida stem sap

Inflammatory diseases remain a significant public health issue, posing challenges in their management due to the adverse effects associated with conventional anti-inflammatory drugs. Exploring medicinal plants as a viable avenue for researching novel anti-inflammatory drugs offers a promising alternative. Jatropha multifida is a well-known traditional plant of the Euphorbiaceae family with undisputed medicinal properties, both haemostatic and antimicrobial. Very little data exists on its anti-inflammatory properties. This study aimed to assess the anti-inflammatory and immunomodulatory properties of Jatropha multifida stem sapThe anti-inflammatory activity of the stem sap was evaluated in vitro by the anti-hemolytic activity assessment method, in vivo by the paw edema test in rats. The immunomodulatory potential was determined using a chemical mediator of inflammation, TNFα. Its antioxidant activity by the DPPH method was evaluated.The results of this study revealed that Jatropha multifida sap with an LC۵۰ of ۰.۶۰۴ mg/mL, is non-toxic and showed a ۶۶.۲۱% higher anti-hemolytic effect than Diclofenac (۲۵.۹۴ %) and Ibuprofen (۴۰ %). It also has a high DPPH inhibitory power (۸۶.۶۱ %) at ۰.۵ mg/mL similar to that of vitamin C. Sap at the concentration of ۸۰۰ mg/Kg has overall the same effects on paw inflammation as Diclofenac and results in a greater decrease in TNFα (۲۸۶.۱۵) at values similar to those of normal control rats.The therapeutic use of this sap in a traditional setting is therefore once again justified due to its proven anti-inflammatory and immunomodulatory properties.

کلیدواژه ها:

Jatropha multifida ، Anti-inflammatory ، rat ، Tnfα