The Efficacy of Cymbopogon nardus (Citronella) Leaf Extract Biolarvicide on the Mortality of Third Instar Larvae of Malaria Vector

Anopheles sp., as vectors of malaria, continue to pose a significant global health challenge. Controlling the population of Anopheles sp. larvae using biolarvicides is considered more environmentally friendly compared to synthetic larvicides. This study assesses the efficacy of biolarvicide derived from Cymbopogon nardus leaf extract on the mortality rates of third-instar larvae of the malaria vector. Employing a true experimental design with a randomized block design (RBD) framework, this study focused on third-instar larvae of Anopheles sp., cultured from naturally sourced eggs, with a total sample of ۶۰۰ larvae. Each experimental group, representing concentrations of ۰%, ۵%, ۱۰%, ۱۵%, ۲۰%, and ۲۵%, contained ۲۵ larvae with four replicates per group. Larvae mortality was recorded after ۲۴ hours. The data were analyzed using Probit analysis, One-way ANOVA, and the Least Significant Difference (LSD) test, with a significance threshold set at α = ۵%. The application of Cymbopogon nardus leaf extract at concentrations of ۵%, ۱۰%, ۱۵%, ۲۰%, and ۲۵% resulted in larval mortality ranging from ۱۵% to ۹۵%. Probit analysis indicated an LC۵۰ value of ۱۰.۰۱۸%. There was a significant difference in mortality rates among different concentrations of the leaf extract (p = ۰.۰۰۰ < ۰.۰۵). Cymbopogon nardus leaf extract biolarvicide is effective in inducing mortality in Anopheles larvae, thereby offering a viable method for controlling the malaria vector population.

کلیدواژه ها:

Biolarvicide ، Cymbopogon Nardus Leaf ، Malaria Vector ، Third Instar Larvae