Biomarker Potential of SLC۴۵A۳ and lncPCAT۱۴ Expression in Prostate Cancer: A Comparative Analysis of Urine Samples from Men with Prostate Cancer and Benign Prostate Hyperplasia

Introduction: Prostate cancer is the second most common cancer globally and is the fifth highest contributor to cancer-related deaths in men. The purpose of this research is to investigate the potential involvement of the SLC۴۵A۳, CHRNA۲, and PCAT۱۴ genes in urine samples taken from men with prostate cancer following a digital rectal examination, compared to those with benign prostatic hyperplasia (BPH).Methods: In this case-control study, ۲۵ post-DRE urine samples of patients with prostate cancer and ۲۵ post-DRE urine samples of patients with BPH were collected. RNA was extracted from the samples, and cDNA synthesis was performed. Real-Time PCR was used to quantitatively analyze the relative expression levels of SLC۴۵A۳, CHRNA۲, PCAT۱۴, and GAPDH. Statistical analysis was conducted using SPSS ۲۴ and GraphPad Prism ۸.۴.۳ software.Results: The findings demonstrated that, in comparison to the BPH group, the expression of SLC۴۵A۳ and PCAT۱۴ was significantly higher in PCa. Furthermore, the expression of CHRNA۲ was not statistically significant (P-value<۰.۱۱). Further analyses showed that SLC۴۵A۳ (P-value<۰.۰۵) and PCAT۱۴ (P-value<۰.۰۵) expression levels were associated with total PSA values and Gleason score of pathological study of biopsies.Conclusion: In future with further studies, SLC۴۵A۳ and PCAT۱۴ genes may be used as diagnostic molecular biomarkers for diagnosis and prognosis of prostate cancer along with PSA and other screening tests.

کلیدواژه ها:

Prostate Cancer ، Benign prostate hyperplasia ، SLC۴۵A۳ ، PCAT۱۴ ، CHRNA۲ ، prostate biopsy