A novel concept: using MgSO۴ as an in vitro anticoagulant in blood samples from dogs and cats and comparing platelet indices with K۲ EDTA

EDTA salts are commonly used as anticoagulants for hematological analysis in humans and pets because they preserve cellular components well. However, a rare drawback is pseudothrombocytopenia (PTCP), caused by binding antibodies to platelet glycoprotein (GP)IIb/IIIa in the presence of EDTA. It is an in vitro artifact without clinical relevance, but failure to diagnose it could lead to unnecessary and expensive diagnostic investigations and dangerous treatments. In ۲۰۱۳, MgSO۴ was found to be an effective anticoagulant for estimating platelet counts in EDTA-induced PTCP (EDTA-PTCP), as it inhibits fibrinogen binding to the GPIIb/IIIa and thromboxane A۲ formation while promoting cAMP formation to block platelet activation. Since ۲۰۱۳, several publications have recommended using MgSO۴ for human platelet counts. However, the potential of this concept in pets has yet to be explored. This article introduces the new concept and compares MgSO۴ with EDTA on the platelet indices in healthy dogs and cats.

کلیدواژه ها:

EDTA ، Dogs ، MgSO۴ ، Pseudothrombocytopenia