Molecular Diagnosis of drug-resistant Mycobacterium Tuberculosis Isolates by IS۶۱۱۰ gene in patients suspected to Tuberculosis in Tehran City

Introduction: Mycobacterium tuberculosis is one of the most dangerous infectious diseases in the world. Determining the diagnosis of M. tuberculosis based on culture and phenotypic tests takes weeks and do not have appropriate methods to evaluate a definitive diagnosis. Therefore, the PCR technique of the IS۶۱۱۰ gene is an accurate, quick, and inexpensive method for the detection of M. tuberculosis. Generally, the purpose of the study is to provide molecular identity strains of M. tuberculosis resistant to first-line drugs (rifampin and isoniazid) detected from patients by PCR-IS۶۱۱۰. Materials and Methods: Of the ۳۰۱۲ Mycobacterium suspected cases, ۶۰ samples of mycobacterium drug-resistant (MDR) were examined by smear, culture, phenotypic tests, and antibiogram. The isolates were identified by IS۶۱۱۰ gene after DNA extraction. Results & Conclusions: Out of ۶۰ collected samples, ۵۴ positive samples from the M. tuberculosis group (۹۰%) and ۶ negative samples (۱۰%) from non-tuberculosis group were reported. Out of ۳۰۱۲ clinical samples, ۱۰۰ samples cultures were positive (۳.۳%), Out of ۵۴ M. tuberculosis were samples, ۳ samples were resistant to rifampin (۵%) and the other samples were MDR (۹۴%). The IS۶۱۱۰ gene is one of the best marker genes for the Mycobacterium complex differentiation. This study showed that PCR-IS۶۱۱۰ technique is a simple and accurate method for identifying drug resistance of clinical isolates of M. tuberculosis in clinical Laboratories. Instead of using phenotypic methods which is time-consuming and have variable results in diagnostic laboratories, it is suggested to use molecular methods directly, especially IS۶۱۱۰ gene sequencing.

کلیدواژه ها:

Molecular detection ، Mycobacterium tuberculosis ، MDR strains ، Molecular detection ، IS۶۱۱۰ gene