The Effectiveness of May Grunwald Giemsa Staining on Tissues Fixed with Date Molasses

Background: Formaldehyde is a reactive chemical that bonds randomly with various cellular elements. This substance, which is also found in the natural structure of the organism, is used in many areas, from industry to household materials, from the production of coatings in dentistry to the fixation of cadavers in laboratories. Formaldehyde is commonly used in laboratories to fix tissues. Objectives: In this study, the staining properties of tissues fixed with date molasses, a natural sugar, using the May-Grunwald-Giemsa (MGG) staining method were compared with the staining properties of tissues fixed with buffered formol-saline.Methods: Liver, spleen, kidney, skin, testicle, small intestine, large intestine, brain, cerebellum, and lung tissue samples of ۴ rats of both genders were used as materials in the study. Each tissue underwent routine histological tissue tracing and was stained with MGG.Results: When all tissues were evaluated anatomically, the general morphology of the tissues showed that the tissue integrity was preserved. There was no color change in the tissues. In addition, tissue hardness was at the required level, supporting previous studies. Histologically, regarding chromatin distribution, nucleus separation and cytoplasm staining, tissues fixed with date molasses and tissues fixed with buffered formal saline showed similar properties, except for the tissues subjected to intense maceration (skin and small intestines). Pale staining was observed in some tissues fixed with buffered formal saline (liver, skin, heart), but the nucleus and cytoplasm structures were better distinguished at higher magnifications. Considering the chromatin distribution, nuclear separation and cytoplasm staining in other tissues (spleen, kidney, testicle, large intestine, brain, cerebellum and lung), tissues fixed with date molasses showed similar properties to tissues fixed with buffered formal-saline.Conclusion: We recommend that researchers interested in the subject prolong the fixation period (۶ months) and compare these results with those of tissues fixed for ۲۴ hours.

کلیدواژه ها:

Buffered formal saline ، Date molasses ، fixation ، histological ، May-Grunwald-Giemsa