Gossypol as Antiacetylcholinesterase in Spodoptera littoralis (Lepidoptera: Noctuidae) Larvae Probed by Kinetic Studies

Introduction: To combat Lepidoptera pests, gossypol may be an alternative to synthetic pesticides. Gossypol may work by inhibiting acetylcholinesterase (AchE) activity, which results in increased pest mortality.Methods: Tested role of gossypol as inhibitor of AchE from Spodoptera littoralis in vivo and in vitro was examined by colorimetric method.Results: The outcomes demonstrated that the suppression of AchE in vivo was dose- and time-dependent. Gossypol showed strong inhibitory activity of AchE with I۵۰ (۰.۲۴ μM) and Ki (۶ μM) values. Michaelis constant (Km) and maximum velocity (Vmax), which are kinetic characteristics indicate the high potency of the inhibitor with the enzyme.In addition, , the kinetic analysis showed a reversible non-competitive inhibition.Conclusion: Based on these results, it is inferred that gossypol is responsible for AchE inhibitory potential from S. littoralis, and thus it can be used as an AchE-targeting insecticide.

کلیدواژه ها:

AChE inhibitor ، allelochemicals ، Enzyme inhibition ، Enzyme kinetics