Reverse Flow Injection Method for Estimation of Hesperidin and Naringenin in Supplements with ۳-Methyl-۲-Benzothiazolinone Hydrazone Hydrochloride

The detection of Hesperidin (HSP) and Naringenin (NAR) in dietary supplements and human urine samples is recommended using a reversed flow-injection analysis (rFIA) technology that is quick, accurate, and highly responsive. Using ۳-methyl-۲-benzothiazolinone Hydrazone Hydrochloride (MBTH) in the presence of sodium periodate as an oxidizing agent, the suggested approach uses oxidative coupling procedures. With a peak absorbance for HSP at ۵۰۷ nm and for NAR at ۴۹۵ nm, a red complex is the resultant product. An extensive analysis of the physical and chemical elements influencing the reaction has been conducted. Between ۲ and ۱۵۰ µg/mL, the calibration curve showed a steady and proportionate relationship. It was found that the detection limits for NAR and HSP were ۰.۹۱۱ µg/mL and ۰.۸۵۶ µg/mL, respectively. For HSP and NAR, the relative standard deviation (RSD) was found to be ۰.۷۹% and ۰.۶۴%, respectively. Drug quantitation was effectively accomplished using the recommended methods.

کلیدواژه ها:

Reverse flow injection ، Flavonoids ، Hesperiden ، Naringenin ، Oxidative Coupling ، ۳-methyl -۲-benzothiazolinone hydrazine hydrochloride