The regulatory roles of Smad۲/۳ protein and SMURF۲ gene expression in granulosa cells of germinal vesicle and metaphase II oocytes in polycystic ovarian syndrome: A case-control study

Background: The impaired functions of granulosa cells (GCs) in the delayed development and immaturity of oocytes have been reported in polycystic ovary syndrome (PCOs). Even with ovarian stimulation, a large number of oocytes in these patients are still in the stage germinal vesicle (GV). Objective: The levels of Smad۲/۳, phosphorylated Smad۲/۳ (P-Smad۲/۳), the expression of SARA, Smad۴, and SMURF۲ genes in the GCs surrounding metaphase II (MII) or GV oocytes in PCOs women were investigated. Materials and Methods: GCs of MII and GV oocytes were isolated from ۳۸ women with PCOs and the expression levels of SARA, Smad۴, and SMURF۲ in surrounding GCs of MII and GV oocytes were determined using reverse-transcription polymerase chain reaction. Also, Smad۲/۳ and P-Smad۲/۳ proteins were determined using western blotting. Results: The expression level of SMURF۲ was significantly higher in GCs surrounding GV oocytes compared with that of GCs encompassing MII oocytes (p < ۰.۰۰۱). At the same time, no significant differences were observed in SARA and Smad۴ expression levels in GCs surrounding GV and MII oocytes. A lower level of P-Smad۲/۳ was also found in GCs GV oocytes compared with GCs of MII oocytes (p < ۰.۰۰۱). Conclusion: It seems that P-Smad۲/۳ plays a role in oocyte development, and the downregulation of this protein is associated with a defect in the maturation of GV oocytes. On the other hand, the upregulation of the SMURF۲ gene also affects the growth process of GCs and the maturation of GV oocytes.

کلیدواژه ها:

Polycystic ovary ، Oocytes ، Granulosa cell. ، تخمدان پلی کیستیک ، تخمک ، سلول های گرانولوزا.