Association between IL-۱۰ rs۱۸۰۰۸۷۲ Polymorphism in the Promoter Region of Human IL-۱۰ Gene and Susceptibility to Rheumatoid Arthritis in the Patients in Southwest of Iran

Aims: Interleukin-۱۰ is an important immunoregulatory cytokine that has an inhibitory effect on the initiation and progression of inflammatory responses in different situations such as autoimmune diseases including rheumatoid arthritis. Polymorphism in IL-۱۰ promoter region affects the expression of this gene. It therefore seems reasonable that these polymorphisms may contribute to rheumatoid arthritis susceptibility in special populations. the aim of this study was to investigate the association between IL-۱۰ rs۱۸۰۰۸۷۲ polymorphism in the promoter region of human IL-۱۰ gene and susceptibility to rheumatoid arthritis in the patients in southwest of Iran. Materials & Methods: The present study is a case-control study conducted among patients with and without rheumatoid arthritis referred to the Mofateh clinic in Yasuj, Iran. Both case (n=۶۴) and control (n=۶۵) groups were selected using purposive sampling and were matched in terms of age, gender and ethnicity. Peripheral blood samples were collected from subjects and rs۱۸۰۰۸۷۲ genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism method. SPSS ۲۶.۰ software was used for statistical analysis of data Findings: The association between the frequency of TT genotypes and the incidence of rheumatoid arthritis was significant (OR=۶.۴۵; ۹۵% CI= ۱.۳۰-۳۲.۰۰; p=۰.۰۲۳). On the other hand, the distribution of the T allele showed a significant relationship with rheumatoid arthritis (OR= ۲.۲۳; ۹۵% CI= ۱.۲۶–۳.۹۷; p=۰.۰۰۶). Conclusion: TT genotype of rs۱۸۰۰۸۷۲ polymorphism is associated with rheumatoid arthritis susceptibility in southwestern Iranian population and can be considered as a risk factor for rheumatism.

کلیدواژه ها:

Rheumatoid Arthritis ، Polymorphism ، Single nucleotide polymorphism