Heracleum persicum L. extract protects gentamicin-induced testicular toxicity

سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 56

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شناسه ملی سند علمی:

JR_AJP-14-5_006

تاریخ نمایه سازی: 3 شهریور 1403

چکیده مقاله:

Objective: The objective of this study was to assess the protective effects of Heracleum persicum L. leaves extract (HPE) against oxidative damage induced by gentamicin (GM) in the testes of rats through biochemical, histopathological, and immunohistochemical approaches. Materials and Methods: Thirty-six male Wistar rats were divided into six groups (n=۶/group) for ۵۰ days. On day ۵۱, the study assessed serum levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone (TT), as well as antioxidant enzyme activity, nitric oxide levels, and various parameters related to testicular tissue (including the ferric reducing ability of plasma (FRAP), thiol, and thiobarbituric acid reactive substances (TBARS) levels). The stereological indices of seminiferous tubules were measured using serial sections of testicular tissue stained with hematoxylin and eosin, while the apoptosis rate of testicular parenchymal cells (p۵۳, Caspase-۳, and Bcl-۲ positive cells) was also determined.Results: In the groups treated with HPE, particularly at ۷۵۰ mg/kg, there was a significant increase (p<۰.۰۵) in LH, FSH, and TT hormone levels,  an enhanced serum antioxidant enzyme activity and significantly reduced (p<۰.۰۵) nitric oxide levels. HPE inhibited the apoptotic pathway involving Bax/p۵۳/Caspase-۳ (significantly decreased (p<۰.۰۵) all three genes), thereby preserving the structure and function of the testicular tissue. Consequently, the number of p۵۳ and Caspase-۳ positive testicular cells decreased significantly (p<۰.۰۵), while the number of Bcl-۲ positive cells increased. Conclusion: HPE demonstrated potential in protecting the function and structure of testis against toxic and oxidative damages.

نویسندگان

Elnaz Khordad

Department of Anatomy, Torbat Heidariyeh University of Medical Sciences, Torbat Heidariyeh, Iran

Mohsen Akbaribazm

Department of Anatomy, Torbat Heidariyeh University of Medical Sciences, Torbat Heidariyeh, Iran

Seyed Moein Hosseini

Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran