Viral Co-Infection Diagnosis in Cervical Samples Using Next Generation Sequencing Technology

Introduction and Aims: The study explores human papillomavirus (HPV), a significant cause of cervical cancer, and its potential coinfections with other viruses like human herpes virus type ۴ (HHV-۴) or Epstein-Barr virus (EBV) and HIV. Next-generation sequencing (NGS) technology is used to analyze viral content in cervical samples from Iraqi women.Methods: Eight cervical samples were collected and processed using Illumina DNA Prep on the iSeq۱۰۰ System. Viral genomic DNA was isolated and libraries prepared for sequencing.Results: MetaviralSPAdes assembled contigs from each sample, which were then blasted against the NT database. HPV۱۶, HHV۴, HIV, and bovine alpha herpesvirus ۵ were among the viruses identified across samples.Conclusion: Viral coinfections identified through NGS underscore their potential impact on cervical health, emphasizing NGS's diagnostic sensitivity for viral infections not typically detected by standard gynecological methods.

کلیدواژه ها:

Viral Co-infection ، Human Papillomavirus Virus (HPV) ، cervical cancer ، Next Generation Sequencing (NGS)