Simultaneous Determination of Chlorhexidine and Triclosan in Some Pharmaceutical Personal Care Products Using Q-Absorbance Ratio Spectra and H-point Standard Addition Methods

Two accurate, rapid, sensitive, inexpensive, and eco-friendly spectrophotometric techniques have been validated for simultaneous determination of chlorhexidine and triclosan. The first method (Q- Absorbance Ratio Spectra method, was applied at ۲۷۲.۸۹ nm (isoabsorptive point) and ۲۵۹.۰۰ nm (λ-max of chlorhexidine) with ۲۸۲.۰۰ nm (λmax of triclosan). The final concentrations for chlorhexidine and triclosan were computed using the Q-equations. The linearity ranges were ۱.۰-۲۰.۰ µg/mL and ۱.۰–۵۰.۰ µg/mL, respectively. The second method, “H-point standard addition method,” was applied at pair of wavelengths (۲۵۱.۰۰ and ۲۷۱.۰۰) nm and (۲۷۲.۰۰ and ۲۸۳.۰۰) nm, respectively. The linearity range for both methods is ۴.۰–۴۰.۰ µg/mL. The recovered values ranged from ۹۶.۹۴-۱۰۰.۰۹% to ۹۶.۳۸-۹۹.۷۶%. The statistical comparisons of the accuracy and precision of the outputs from the suggested methods were compared using, Student's t-test, F-test, and One-way ANOVA. The proposed methods were successfully applied for the determination of chlorhexidine and triclosan in various toothpaste samples. Lastly, an assessment of the environmental impact of the proposed techniques was conducted utilizing the green analytical procedure index and analytical eco-scale approaches as a measure of the "greenness" profile.

کلیدواژه ها:

Chlorhexidine ، Greenness measurement ، H-point ، Q-absorbance ، Triclosan