Evaluating the Anti-Migratory Effect of Fucoxanthin in Human Cisplatin-Resistant Ovarian Cancer Cells

Background & Objective:  Ovarian cancer ranks among the most prevalent gynecologic cancers in women with the majority of patients experiencing a recurrent type and developing treatment resistance. Resistance to chemotherapy drugs has been observed to trigger epithelial-mesenchymal transition (EMT), which leads to the acquisition of metastatic properties. Thus, targeting the molecular mechanism of EMT may lead to novel interventions against metastatic disease. In this research, the suppressive impacts of fucoxanthin, as a natural compound, were examined on cell migration and expression of key EMT-related markers in ovarian cancer cells which were sensitive and resistant to cisplatin.  Materials & Methods:  To determine the non-toxic concentrations of fucoxanthin for migration assay, the MTT assay was conducted. To assess the anti-migratory capacity of fucoxanthin in ovarian cancer cells which were sensitive and resistant to cisplatin, a wound-healing migration test was done in the presence of non-toxic concentrations of fucoxanthin. RT-qPCR was utilized to examine the impact of fucoxanthin on the mRNA expression of E-cadherin, vimentin and α- SMA (as EMT-related markers) in ovarian cancer cells. Results:  Fucoxanthin at concentrations of (۱ and ۲.۵ μM) downregulated the expression of α–SMA in cisplatin-resistant ovarian cancer cells and inhibited migration in ovarian cancer cell lines. Conclusion: Fucoxanthin exhibited remarkable efficacy in inhibiting migration in in ovarian cancer cells which were sensitive and resistant to cisplatin. However, more research is required to ascertain the clinical advantages linked to utilizing fucoxanthin in the treatment of cisplatin-resistant ovarian cancers.

کلیدواژه ها:

Fucoxanthin ، Ovarian cancer ، EMT marker ، Cell migration