Effect of Surface Modification on Viability of L۹۲۹ Cells on Zirconia Nanocomposite Substrat

Abstract Introduction: Zirconia bioceramic can be considered for metallic replacement in dental implant applications. A proper method of surface modification may promote better osseointegration.Methods: In study evaluated viability of fibroblast cell following surface treatment. Therefore, viability L۹۲۹ cells were characterized using MTT assay and scanning electron microscopy.Results: The viability assessment determined significant differences A-Y-TZP۲۰ without surface treatment as compared to laser surface treatment (B), laser surface treatment + hydroxyapatite-yttrium stabilized tetragonal zirconia nanocomposite coat (C) and control. This study demonstrated that L۹۲۹ cells approximately proliferated and spread on A-Y-TZP۲۰ nanocomposite disk in laser surface treatment(B), Laser surface treatment + hydroxiapatite-yttrium stabilized tetragonal zirconia nanocomposite coat (C) groups similar to control group.Conclusion: Laser surface treatment showed positive effect on the viability of L۹۲۹ cells. Keywords: Alumina-yttrium stabilized tetragonal zirconia nanocomposite Femtosecond laser MTT assay Endosseous dental implant.