The Effect of Exposure to Hypoxia during Resistance Training on the Concentration of PAX۷ and PGC-۱α Proteins in Fast-Twitch Muscle Tissue of Diabetic Rats Fed a High-Fat Diet

Introduction: Myopathy caused by diabetes can accelerate the disease process in diabetic people. Myopathy has important indicators in muscle tissue related to regeneration and intracellular metabolism in skeletal muscles. The current study aimed to evaluate the effect of eight weeks of resistance training in hypoxia on the content of PAX ۷ and PGC-۱α proteins in the gastrocnemius muscle of type ۲ diabetic rats. Methods: This experimental study was conducted on ۲۴ male Wistar rats for six weeks after induction of type ۲ diabetes. Rats were divided into three groups: healthy control (HC), diabetic control (DC), and hypoxia group (HPX). Resistance training was applied for eight weeks under oxygen deficiency conditions in the groups of resistance training in hypoxia. The tissue sample was taken from the biceps muscle after finishing the exercises and evaluated to measure the concentration of PAX۷ and PGC-۱α proteins. The results were analyzed by one-way analysis of variance at a significance level α ≤۰.۰۵. Results: There was a significant difference in PAX۷ and PGC-۱α proteins between the research groups (P=۰.۰۰۰۱). Induction of diabetes led to a significant decrease in PAX۷ compared to the control group. PGC۱-α protein levels also decreased significantly in the diabetes induction group compared to the control group (P=۰.۰۰۰۱). However, exposure to hypoxia did not change the gene expression values of this variable compared to the diabetic patient group (P=۰.۴۵۱). Conclusion: Exposure to temporary and passive hypoxia can be considered as a suggested strategy to improve indicators related to type ۲ diabetes in humans.

کلیدواژه ها:

Diabetic myopathy ، Hypoxia ، PAX۷ ، PGC-۱α