Biogenic Synthesis of Papain Conjugated Copper Metallic Nanoparticles and Its Antibacterial and Antifungal Activity

Biogenic synthesis of papain-conjugated copper metallic Nanoparticles and their antibacterial and antifungal activities Papain metallic conjugated nanoparticles (Papain-CuNPs) were synthesised using Papain and CuSO۴.۵H۲O. Papain-CuNPs were characterized using UV-visible spectroscopy, FT-IR, HR-TEM, XRD, FE-SEM, zeta potential, and a zeta sizer. The antibacterial activity of papain-CuNPs against human infectious microorganisms (Citrobacter spp, Pseudomonas aeruginosa and Candida albicans) was investigated. The mechanism of action of papain-CuNPs was evaluated using FE-SEM and HRTM. UV spectroscopy confirmed the plasma resonance (SPR) at ۶۷۹ nm, which indicated the formation of papain-CuNPs. The FT-IR spectrum absorbance peaks at ۳۹۲۷, ۳۸۶۵, ۳۸۴۲, ۳۳۶۳, ۲۹۷۸, and ۲۹۰۰ cm-۱ indicate the presence of O-H and N-H of the secondary amine, and peaks at ۱۶۴۳ and ۱۵۷۲ cm-۱ represent C=O functional groups in Papain-CuNPs. EDAX analysis confirmed the presence of copper in the papain-CuNPs. The zeta potential (-۴۲.۶ mV) and zeta size (۹۹.۶۶ d. nm) confirmed the stability and size of the nanoparticles. XRD confirmed the crystalline nature of the papain-CuNPs. FE-SEM and HRTM showed an oval structure, and the nano particles' ۱۶.۷۱۲۴۴–۳۴.۸۴۷۹۳ nm. The synthesized papain-NPs showed significant antibacterial activity against clinical P. aeruginosa (۱۵ mm). MIC ۱۲۵ µg/ml) showed bactericidal activity against P. aeruginosa and the mechanism of action of Papain-NPs was confirmed using an electron microscope by observing cell damage and cell shrinking. Papain-CuNPs have significant antibacterial activity and are thus used in the treatment of P. aeruginosa infections

کلیدواژه ها:

Papain ، Copper sulphate ، Nanoparticles ، SE-SEM ، HRTEM