Effects of dose-dependent chronic caffeine consumption in a rat burn wound model: Histopathological and immunohistochemical evaluation

Objective(s): Using histopathological and immunohistochemical methods, we aimed to examine the dose-dependent effects of chronic caffeine consumption on the recovery of burn wounds in an in vivo rat model.Materials and Methods: Forty-five rats were randomly assigned to a high-dose group (۲۰ mg/kg per day for eight weeks; n=۱۵), a low-dose group (۱۰ mg/kg per day for eight weeks; n=۱۵), or a control group (n=۱۵). The burn model was created in rats. The groups were separated into three subgroups (n=۵) based on the day after injury (۷th, ۱۴th, or ۲۱st day). The wound area, wound closure percentage, and histopathological and immunohistochemical reactivity were evaluated.Results: Successful wound healing was noted in rats treated with low doses of caffeine, similar to the control group. Pathology revealed low re-epithelization, low inflammation, and high granulation in the high-dose group. In addition, there was a significant difference between the control and high-dose groups regarding the immunohistochemical reactivity of αVβ۳ integrin, VEGF, and MMP-۹ (P<۰.۰۵).Conclusion: We demonstrated that chronic caffeine consumption in rats adversely affects the recovery process of wounds in a dose-dependent manner. This effect may occur through delayed wound healing via the molecules MMP-۹, αVβ۳ integrin, and VEGF. Treatment that modulates these molecules can lead to enhanced and quicker recovery of damaged skin in coffee lovers.

کلیدواژه ها:

Caffeine ، Integrin ، MMP-۹ ، VEGF ، Wound healing