Analysis of Matrix Metalloproteinase-۹ Promoter Region Activity and Association Analysis of Promoter Region SNPs with Lactation Traits in Dairy Goats

Matrix Metalloproteinase-۹ (MMP۹) degrades the Extracellular Matrix (ECM), participates in mammary gland remodeling, and inhibits mammary epithelial cell apoptosis in goats. To investigate the transcriptional regulatory mechanism of the MMP۹ promoter region, we analyzed the expression pattern of MMP۹ in dairy goats by qRT‒PCR and cloned the promoter region by PCR. Deletion analysis indicated that the MMP۹ gene core promoter region was located upstream of the transcription start site in the -۷۱۵ bp to -۹۲۶ bp region. We predicted three Specificity protein ۱ (Sp۱) binding sites in the MMP۹ core promoter region, and performed targeted mutations on these three sites. The c.۱۸۶۳ G> A mutation in the MMP۹ gene increased the promoter transcriptional activity and may be associated with an additional Serum Response Factor (SRF) transcription factor-binding site. Association analysis revealed that c.۱۸۶۳ G> A was significantly associated with milk fat percentage in dairy goats, which was significantly higher in goats with the AG genotype (۴.۷۱±۰.۰۲%) than in goats with the GG genotype (۴.۶۱±۰.۰۵%). This study lays a foundation for subsequent analysis of the transcriptional regulatory mechanism of MMP۹ and exploration of its biological functions.

کلیدواژه ها:

Laoshan dairy goat ، MMP۹ ، SNP ، Sp۱.