Green synthesis, characterization, and evaluation of antimicrobial and antioxidant activities of CuO, Co۳O۴, and CuO-Co۳O۴ Nano system using Moringa stenopetala plant leaf extracts

Objective(s): The purpose of this study was to synthesize copper oxide nanoparticles (CuO NPs), cobalt oxide nanoparticles (Co۳O۴ NPs), and CuO-Co۳O۴ nano system using Moringa stenopetala plant leaf extract. These nanoparticles were specifically developed for their antioxidant and antibacterial properties.Materials and Methods: The nanoparticles were synthesized by a green synthesis approach using M. stenopetala leaf extract. Comprehensive characterization using spectroscopic techniques such as X-ray diffraction (XRD), UV-visible spectroscopy, scanning electron microscopy (SEM), Fourier transform infrared (FT-IR), and thermogravimetric analysis (TGA) was executed.Results: UV-Vis analysis revealed an energy bandgap of ۳.۳۲ eV for CuO NPs, ۳.۳۶ eV for Co۳O۴ NPs, and ۳.۶۶ eV for CuO-Co۳O۴ nano system. XRD analysis revealed that CuO NPs have a monoclinic crystal structure with an average crystallite size of ۱۱.۵۹ nm, whereas Co۳O۴ NPs have a cubic crystal structure with an average crystallite size of ۱۲.۲۵ nm. The CuO-Co۳O۴ nano system exhibited an average crystal size of ۹.۵۳ nm. The SEM images showed inhomogeneous composition and large granular particles of CuO NPs, inhomogeneous cubic shape and size with encapsulation and hydrogen bonding aggregation of Co۳O۴ NPs, and particle heterogeneity. The CuO-Co۳O۴ nano system. FT-IR analysis confirmed the presence of bioactive molecules such as flavonoids, tannins, terpenoids, steroids, glycosides, saponins, and phenols that actively participate in the synthesis process.Conclusion: The synthesized CuO-Co۳O۴ nano system showed superior inhibitory effects against the selected bacterial strains compared to CuO NPs and Co۳O۴ NPs. Furthermore, they showed excellent antioxidant activity. These results highlight the significant potential of CuO-Co۳O۴ nano system for a wide range of applications due to their remarkable bacterial inhibition and radical scavenging properties.

کلیدواژه ها:

Antimicrobial activity ، Antioxidant activity ، Bacteria ، Biosynthesis ، Nano system ، Phytochemicals