Culture of stem cells on carbon nanotubes- nanoporous alumina substrates

Alumina substrates are one of the commonly used scaffolds applied in cell culture, but in order to preventformation of biofilm on the alumina substrate, these substrates are modified with carbon nanotube. Thealumina substrate was made by a two-step anodization method and was then modified with carbon nanotubesby simple chemical reaction. The substrates were characterized with FTIR, SEM, EDX, ۳D laser scanningdigital microscope, contact angle (CA) and surface free energy (SFE). To determine how this modificationinfluences the reduction of biofilm, biofilm of two various bacteria, Escherichia coli (E.coli) andStaphylococcus aureus (S. aureus), were investigated. The biofilm on the modified substrate decreased due tothe presence of carbon nano- tubes and increased antibacterial properties. Dental pulp stem cells (DPSCs)were cultured onto flat alumina (FA) and nanoporous alumina-multiwalled carbon nanotubes (NAMC)substrates to examine how the chemical modification and surface topography affects growth of DPSCs.Cell attachment and proliferation were investigated with SEM and Presto Blue assay, and the findings showthat the NAMC substrates are suitable for cell culture.

کلیدواژه ها:

alumina scaffold ، dental pulp ، biofilm ، adhesion ، proliferation