Assessment of genetic diversity in different types of tobacco using molecular markers

Information about the amount of genetic diversity in the germplasm and genetic relations of genotypes is essential for analyzing and designing breeding programs and could be used for assisting in genetic identification and improvement of the germplasm. In this research, the genetic diversity of ۴۸ genotypes of the flue-cured, burley, and oriental types of tobacco was investigated by ۱۲ ISSR, ۱۰ SSR, and five IRAP primers. The maximum number of bands was observed in the UBC۸۲۵, RTR-۱۰, and mixed primer UBC۸۱۷ + UBC۸۲۶ with ۱۷ bands, UBC۸۱۷ with ۱۶ bands, and RTR-۸ with ۱۵ bands. UBC۸۲۴ with ۱۰ and UBC۸۲۳ with ۱۱ bands showed the minimum number of bands among ISSR and IRAP primers, and PT۳۰۰۴۴ and PT۳۰۰۴۶ primers with two alleles showed the minimum number of alleles among SSR markers. In ISSR and IRAP, the observed percentage of polymorphism ranged from ۷۶.۹۲% for RTR-۱ and RTR-۷ to ۹۴.۱۱% for RTR-۱۰, and in SSR markers, it was ۱۰۰%. The average polymorphism percentage was ۹۰.۷%, which indicated suitable genetic diversity among the tobacco genotypes. The polymorphism information content ranged from ۰.۳۱ to ۰.۵ with an average of ۰.۴۲. The average diversity of Nei and Shannon indices were ۰.۳۹ and ۰.۵۸, respectively. Cluster analysis by the UPGMA method classified the ۴۸ tobacco genotypes into five groups, containing ۵, ۱۲, ۱۰, ۶, and ۱۵ genotypes, respectively. Principal coordinate analysis showed that the ۱۴ first components could explain ۵۱.۴۹% of the total variance. The primers used in this study had high efficiency, which can be used to study the genetic diversity in tobacco.

کلیدواژه ها:

burley ، flue-cured ، IRAP ، Oriental ، SSR