Effects of xenostrogen treatment (۴-Nonylphenol, Bisphenol A) on vitellogenin expression in juvenile Cyprinus carpio

The aim of this study was to examine some potential effects of ۴-Nonylphenol (۴-NP), Bisphenol A (BPA), and mixture of them on common carp, Cyprinus carpio and demonstrate induction of vitellogenin (Vtg) as a biomarker for screening aquatic ecosystems. These compounds with other estrogenic chemicals may be responsible for disrupting effects observed in fish. A total of ۱۰۰ juvenile carp were treated experimentally with ۱۷β-estradiol (E۲), and increasing doses of ۴-NP, BPA and also mixture of them. Then the plasma Vtg levels were measured using indirect competitive ELISA. Results showed a significant (P<۰.۰۵) increase in Vtg levels of exposed carps with E۲, maximum ۴-NP, BPA and their mixture. The group treated with E۲ showed high induction (۴۹۰%) with respect to controls. The groups treated with maximum dose of ۴-NP and BPA showed also induction of Vtg, whereas the group treated with their mixture (۳ mg kg−۱ b.w. ۴-NP and ۰.۰۱ mg kg−۱ b.w. BPA) showed the highest induction (۲۱۲۶%). Finally, results showed that mixture of ۴-NP and BPA exhibited stronger (synergistic) effects. So that, large scale monitoring of estrogenic effects caused by contamination may be considered as a biomarker in urban and industrial effluents.

کلیدواژه ها:

Vitellogenin (Vtg) ، Cyprinus carpio ، ۱۷β-Esteradiol (E۲) ، ۴-Nonylphenol (۴-NP) ، Bisphenol A (BPA) ، ELISA