Effects of acute crude oil exposure on basic physiological functions of Persian sturgeon, Acipenser persicus

Hematological parameters are suitable biomarkers for evaluating the potential risk of the chemicals. The aim of this study was to investigate of acute crude oil exposure on basic physiological functions of Persian sturgeon, Acipenser persicus. ۲۱۰ juvenile Persian sturgeon (۹.۴ ± ۱g) were supplied by the Rajaei fish farm in Mazandaran Province, Iran.  Juveniles were exposed to the crude oil (۱۵, ۱۶, ۱۷, ۱۸ and ۱۹ ppm). The ۹۶h-LC۵۰ were detected under static condition by SPSS software. Hematological and biochemical parameters were compared between control group and treatment exposed to ۹۶h-LC۵۰. The median lethal concentration was ۱۶.۵ ppm in ۹۶ h toxicity test. WBC, RBC, Hb and MCHC decreased, while MCV, MCH and PCV were significantly higher in the treatment which exposed to LC۵۰ concentration (P<۰.۰۱). Results of differential leukocyte count showed that after treatment with LC۵۰ concentration, neutrophils and monocytes increased, while lymphocytes and eosinophils decreased (P<۰.۰۱). Biochemical parameters showed an increase in serum glucose (p<۰۰۱). Other parameters including total protein, ALT, AST, ALP and LDH enzymes decreased in treatment group significantly (p<۰.۰۱). Our results provides evidences that crude oil may have disruptive action on the erythropoietic tissue which may be due to its influence on the viability of the cells. Crude oil also inhibited all of the enzymes activities leading to hyperglycemia due to stress inoculation.

کلیدواژه ها:

Acute exposure ، Biochemical parameters ، Crude oil ، Hematology ، Persian Sturgeon