Isolation and Optimization of Tobacco Decomposing Bacillus and Lactobacillus Sp.

Three bacteria from a tobacco industry effluent and one from a sewage sample were isolated on a medium comprising of ۱% tobacco powder as sole source of nutrients. Bacteria isolated from the industrial waste water were identified as Bacillus cereus, B. alvei and B. circulans. While, Lactobacillus sp. was isolated from the sewage sample. The bacteria were optimized for various growth conditions in a medium comprising of aqueous extract of ۱% tobacco powder in MIII. Provision of ۰.۱% molasses (MIII) enhanced growth of B.cereus as compared to its cultivation in MII. Growth of B.cereus up to two weeks in molasses supplemented ۱% tobacco powder (MIV) medium resulted into retroprogressive decreases in total suspended matter, ash content and total organic matter. So that at the last sampling period these parameter reduced up to ۹۲%, ۸۲% and ۴۲%, respectively as compared to control values. The bacterial isolates in general and the B.cereus practically appear promising for designing in situ biotreatment plants for tobacco processing units and are likely to add in reducing environmental contamination originating from tobacco allied toxic compounds.   REFERENCES Bejankiwar, R.S. (۲۰۰۲) Electrochemical treatment of cigarette industry waste water feasibility study. Water. Res. ۳۶, ۴۳۸۶-۴۳۹۰ Campbell, R.C. (۱۹۸۹). Statistics for biologist. Cambridge University Press Cambridge. Civilini, M, Domenis, C, Sebastianutto, N, Bertoldi, M. (۱۹۹۷). Nicotine decontamination of tobacco agro-industrial waste and its degradation by microorganisms’ Waste. Manag . Res. ۱۵, ۳۴۹-۳۵۸  Clarke, A.B., Stanley, J. (۱۹۶۴). Process for selective extraction of alkaloid. United States Patent No ۳, ۳۱۹-۴۳۵ De Lucas, A, Canizares, P, Garcia, M.A, Gomez, J, Rodrigez, J.F. (۱۹۹۸) .Recovery of nicotine from aqueous extracts of tobacco wastes. Indust. Enging. Chem. Res .۳۷, ۴۷۸۳-۴۷۹۱.  Gorrod, J.W ,Jacob, P. (۱۹۹۹). Analytical determination of nicotine and related compounds and their metabolites Part. Environ. Tobacco. Products. ISBN: ۰-۴۴۴-۵۰۰۹۵-۲, Elsevier, ۷۳۲ p. Gravely, L.E, Geiss, V.L, Newton, R.P. (۱۹۷۷). Process for maximizing the growth and nicotine degrading activity of microorganisms United States Patent No .۴.۰۱۱.۱۴۱ Gupta, P.K. (۲۰۰۰). Methods in environmental analysis water soil and air .AGROBIOS (INDIA) Behind Nasarani Cinema Chopasani Road Jidhpur. Holt, J.G, kriey, N.R, Sneath, P.H.A, Staley, J.T, Williams, S.T.(۱۹۹۴). Bergeys´s manual of determinative bacteriologyLippincott Williams and Wilkins, Philadelphia, USA. pp. ۵۶۲-۵۶۸ Ireland, M.S, Larson, T.M , Moring, T.M. (۱۹۸۰). Nicotine transfer process. United States Patent No ۴.۲۱۵.۷۰۶ Konenan ,E.W, Allen, S.D. Janda, W.M. Schree, Kenberger ,P.C. Winn, Jr. W.C. (۱۹۹۷). Lippincot Williams and Wilkins ۵۳۰ Walnur street, Philadeiphia, PA ۱۹۱۰۶ -۳۶۲۱ USA. Meher, K.K, Panchwagh, A.M, Rangrass, S ,Gollakota, K.G. (۱۹۹۵). Biomethanation of Tobacco. Waste. Environ. Pollut. ۹۰, ۱۹۹–۲۰۲. Munari, M. (۱۹۸۶). Quantitative Determination of Nicotine Content in Protein Extracted from Tobacco.Tobacco Journal International, ۲, ۱۲۸– ۱۳۲. Saunders, J.A , Blume, D.E. (۱۹۸۱). Quantitation of major tobacco alkaloids by high performance liquid chromatography. Chromatog. ۲۰۵, ۱۴۷–۱۵۴. Sax, N.I, Lewis, R.J. (۱۹۸۹). Dangerous properties of industrial materials. New York USA:۲۴۸ Sponza,D.T. (۲۰۰۲). Toxicity studies in a tobacco industry biological treatment plant. Water, Air and Pollution, ۱۳۴, ۱۳۷-۱۶۴.

کلیدواژه ها:

Tobacco effluents? biotreatment ، Tobacco detoxifying bacteria ، Water pollution