In Vitro Inhibition of Growth in Saprolegnia sp. Isolated

Saprolegnia is one of? the? ?most important agents decreasing the eggs survival rate in sturgeon hatcheries. There are some chemical substances for controlling the fungal infection of eggs. In this study, an attempt was made to introduce a ?germ negative bacterium, Pseudomonas aeroginosa (PTCC۱۴۳۰)(Persian Type Culture Collection) as a biocontrol??ling agent of? ?water mold. Saprolegnia was isolated from the eggs of some infected Persian sturgeon, Acipenser persicus? in a sturgeon hatchery and then ?was purified. P. aeroginosa was cultured in Potato dextrose Agar (PDB) media and then was prepared in ۵ concentrations (۱۰۳,۱۰۴,۱۰۵,۱۰۶and۱۰۷cfu.ml-۱) while challenging with fungi in petri dishes under laboratory conditions. The results showed that by increasing the concentration of the bacteria in plates, ???hyphal growth of the fungi was reduced. The ?highest concentration of P. aeroginosa concentration (۱۰۷) roughly stopped the ?fungi growth and the Minimum Inhibitory Concentration (MIC) was ۱۰۴cfu.m-l. Results? in this study implied the potential?? of? P. aeroginosa (PTCC۱۴۳۰) as a biological agent in controlling saprolegniosis.

کلیدواژه ها:

Biocontrol ، Persian sturgeon eggs ، Pseudomonas aeroginosa ، Saprolegniasis