The Relative Separation of Lunasin Peptide from Soya Protein and Checking Its Characteristics with Computational Tools (Based on Bioinformatics Findings)

سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 182

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شناسه ملی سند علمی:

JR_IJHES-2-1_003

تاریخ نمایه سازی: 12 خرداد 1403

چکیده مقاله:

Bioactive peptides are small peptides that can exhibit various properties including anticancer properties. Lunasin peptide is a ۴۳-amino acid peptide isolated from soy protein that has anticancer properties. In this study, soy protein was enzymatically hydrolyzed and has some properties similar to lunasin in in- silico . item It was checked The present study used commercial soybeans and soybean meal. In this research, the trypsin enzyme has acted on the substrate and the water quality has been measured. Protein extracted water was analyzed using methods such as electrophoresis and Bradford test to measure the quantity of protein.In the second phase of the research, the properties of Lunasin were investigated using computational toolsThe quality of water quality for commercial soybeans and soybean meal was ۴.۱۳ and ۳.۸۳, respectively. It was also determined by electrophoresis analysis that the peptides similar to lunasin were derived from soybean by trypsin enzyme. Bioinformatics results showed that acid Amines That At sustainability Role have, able change are. Results sign gave That this Peptide has Capabilities Anti cancerous Is That Maximum Score ۸۷ % receive done Is .Bioactive peptides are small peptides that can exhibit various properties including anticancer properties. Lunasin peptide is a ۴۳-amino acid peptide isolated from soy protein that has anticancer properties. In this study, soy protein was enzymatically hydrolyzed and has some properties similar to lunasin in in- silico . item It was checked The present study used commercial soybeans and soybean meal. In this research, the trypsin enzyme has acted on the substrate and the water quality has been measured. Protein extracted water was analyzed using methods such as electrophoresis and Bradford test to measure the quantity of protein. In the second phase of the research, the properties of Lunasin were investigated using computational tools The quality of water quality for commercial soybeans and soybean meal was ۴.۱۳ and ۳.۸۳, respectively. It was also determined by electrophoresis analysis that the peptides similar to lunasin were derived from soybean by trypsin enzyme. Bioinformatics results showed that acid Amines That At sustainability Role have, able change are. Results sign gave That this Peptide has Capabilities Anti cancerous Is That Maximum Score ۸۷ % receive done Is .

نویسندگان

Fatemeh safakhah

۱-Masters student in microbial biotechnology /Department of Biotechnology, Faculty of Biological Sciences/Alzahra University

Mahboobeh Zarrabi

Assistant Professor of Biophysics/ Department of Biotechnology, Faculty of Biological Sciences/Alzahra University

Fakhri Sadat Hosseini

Assistant Professor of Biotechnology/ Department of Biotechnology, Faculty of Biological Sciences/Alzahra University

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  • • Abel, S. D. A., & Baird, S. K. (2018). ...
  • • Aleksis, R., Jaudzems, K., Muceniece, R., & Liepinsh, E. ...
  • • Andersen, C. A. F., Palmer, A. G., Brunak, S., ...
  • • Ashaolu, T. J., Yantiam, N., & Yupanqui, C. T. ...
  • • Chiangjong, W., Chutipongtanate, S., & Hongeng, S. (2020). Anticancer ...
  • • Dai, Y., Cai, X., Shi, W., Bi, X., Su, ...
  • • Daliri, E. B.-M., Oh, D. H., & Lee, B. ...
  • • de Souza, S. M. A., Fernandes, T. V. A., ...
  • • Esteve, C., Marina, M. L., & García, M. C. ...
  • • Fernández-Tomé, S., Sanchón, J., Recio, I., & Hernández-Ledesma, B. ...
  • • Garnier, J., Osguthorpe, D. J., & Robson, B. (1978). ...
  • • Gonzalez de Mejia, E., Castañeda-Reyes, E. D., Mojica, L., ...
  • • Hernandez-Ledesma, B., Hsieh, C.-C., & Ben, O. (2009). Lunasin, ...
  • • Hoskin, D. W., & Ramamoorthy, A. (2008). Studies on ...
  • • Huang, Y., Wang, X., Wang, H., Liu, Y., & ...
  • • Jahangirian, H., Kalantari, K., Izadiyan, Z., Rafiee-Moghaddam, R., Shameli, ...
  • • Jeong, H. J., Jeong, J. B., Hsieh, C. C., ...
  • • Kawaguchi, K., Han, Q., Li, S., Tan, Y., Igarashi, ...
  • • Kruger, N. J. (2009). The Bradford method for protein ...
  • • Kong, X., Guo, M., Hua, Y., Cao, D., & ...
  • • Kyte, J. (2006). Structure in protein chemistry. Garland Science. ...
  • • Lafarga, T., Álvarez, C., Bobo, G., & Aguiló-Aguayo, I. ...
  • • Maddocks, O. D. K., Athineos, D., Cheung, E. C., ...
  • • Pimentel, F. B., Alves, R. C., Harnedy, P. A., ...
  • • Rodrigues, E. G., Dobroff, A. S., Taborda, C. P., ...
  • • Roy, S., & Teron, R. (2019). BioDADPep: a bioinformatics ...
  • • Ruth, T. K., Rumble, J. N., Lamm, A. J., ...
  • • Rutherfurd, S. M. (2010). Methodology for determining degree of ...
  • • Sharma, P., Kaur, H., Kehinde, B. A., Chhikara, N., ...
  • • Shoombuatong, W., Schaduangrat, N., & Nantasenamat, C. (2018). Unraveling ...
  • • Singh, A., Kaushik, R., Mishra, A., Shanker, A., & ...
  • • Suarez-Jimenez, G.-M., Burgos-Hernandez, A., & Ezquerra-Brauer, J.-M. (2012). Bioactive ...
  • • Tyagi, A., Tuknait, A., Anand, P., Gupta, S., Sharma, ...
  • • Variyar, P. S., Limaye, A., & Sharma, A. (2004). ...
  • • Vijayakumar, S., & Ptv, L. (2015). ACPP: a web ...
  • • Vuister, G. W., Fogh, R. H., Hendrickx, P., Doreleijers, ...
  • • Vuyyuri, S. B., Shidal, C., & Davis, K. R. ...
  • • Yang, Q.-Z., Wang, C., Lang, L., Zhou, Y., Wang, ...
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