Effects of Uncultured Adipose Derived Stromal Vascular Fraction on Tendon Healing in Rabbits: A Histological and Immunohistochemical Study

سال انتشار: 1388
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 111

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شناسه ملی سند علمی:

JR_IJVS-4-1_003

تاریخ نمایه سازی: 8 خرداد 1403

چکیده مقاله:

Objective- To evaluate the potential effects of uncultured adipose-derived stromal vascular fraction on tendon healing.Design- Prospective descriptive study.Animals- Twenty-five adult male New Zealand white rabbits, weighing ۲.۵-۳.۰ kg were used. Five rabbits were used as donors of adipose tissue and the rest were divided into control and treatment groups.Procedures- The injury model was unilateral complete tenotomy through the middle one-third of deep digital flexor tendon. Immediately after suture repair, either fresh stromal vascular fraction from enzymatic digestion of adipose tissue or placebo was intratendinously injected at tendon stumps in treatments and controls, respectively. Immobilization with cast was continued for two weeks after surgery. Animals were sacrificed at three and eight weeks after surgery and tendons underwent histological and immunohistochemical evaluations.Results- By histology, superior fibrillar organization and remodeling of neotendon were observed in response to the treatment at both time points. As the main finding of immunohistochemistry, treatment at the later time point resulted in significant increase in collagen I and decrease in collagen III expression compared to controls.Conclusions and clinical Relevance- The present study showed that intratendinous injection of uncultured adipose-derived stromal vascular fraction resulted in improved structural properties of tendon repairs and it could be an effective modality for treating tendon injury.

نویسندگان

Mehdi Behfar

Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Farshid Sarrafzadeh-Rezaei

Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Rahim Hobbenaghi

Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

Nowruz Delirezh

Department of Cellular and Molecular Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran

Bahram Dalir-Naghadeh

Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran