Histhopathologic Evaluation of Curative Impact of Aloe vera L. Fresh Gel on Healing of Eexperimental Infected Full-Thickness Open Wounds Induced with Staphylococcus aureus in Dogs

Objective- to consider the influence of Aloe vera L fresh gel on healing process histopathologically.Design- Experimental studyAnimals- Five adult male mixed breed dogs aged ۲-۴ yearsProcedures- Eight symmetrical full-thickness wounds were surgically created on the back of all five dogs under general anesthesia and sterile condition. After wound creation, ۱ ml fluid containing ۱۰۵ CFU of S. aureus was inoculated on each wound. Right wounds were covered with ۱ ml Aloe vera fresh gel whereas the left wounds were not received any therapeutic material. Wounds’ biopsies were assessed on days ۷, ۱۵, ۲۱ and ۲۸ in treatment and control groups.Results- In microscopic examinations density of collagen fibers in the superficial (P = ۰.۰۳۹) and deep (P = ۰.۰۴۲) sections of ۲۸-days old wounds were significantly higher in treatment group compared to control group. Also these fibers had more diameter and better alignment in treatment group. No significant differences (P > ۰.۰۵) were seen in other indices including fibrocytes, fibroblasts and inflammatory cells between treatment and control wounds.Conclusions and Clinical Relevance- It seems that Aloe vera in addition to antibacterial effects, probably exerts its main effects on the characteristics of collagen fibers. These positive effects could cause the improvement of quality and quantity of collagen fibers and their structures.

کلیدواژه ها:

Aloe vera ، Full-thickness wounds ، Infected wound ، Staphylococcus aureus