PEG-Based Clotrimazole Microparticles Improved Ischemia-Reperfusion Injury in Rat Testicular Torsion and Detortion Model: Evaluation of Tissue Oxidative Stress

A rat testis model was used to assess effects of polyethylene glycol (PEG) clotrimazole microparticles on ischemia-reperfusion injury. Forty-eight healthy male Wistar rats were included and randomized into six investigational groups (n = ۸): Group SHAM: Merely laparotomy was implemented. Group ISCHEMIA: Merely a ۳-hour interval ischemia was done. Group I/R: A ۳-hour interval ischemia, three-hour reperfusion for left testis, and one-week reperfusion for right testis were done and ۲۰ µL normal saline was administered intraperitoneally (IP) ۳۰ min before termination of ischemia. Group I/R/PEG: The same as group I/R as well as ۲۰ µl PEG (IP) ۳۰ min before termination of ischemia. Group I/R/CLTMZLMP: The same as group I/R and ۲۰ µL (۱۰ mg/kg) of clotrimazole microparticles (IP) ۳۰ min before termination of ischemia. Group I/R/PEG-CLTMZLMP: The same as group I/R and ۲۰ µL PEG-based clotrimazole microparticles (IP) ۳۰ min before termination of ischemia. Evaluations were based on analyses of biochemical assays. PEG-based clotrimazole microparticles improved enhanced antioxidant activity (p < ۰.۰۵). PEG-based clotrimazole microparticles could be helpful in minimizing ischemia-reperfusion injury in testicular tissue exposed to ischemia.

کلیدواژه ها:

Clotrimazole microparticles ، PEG ، ischemia-reperfusion ، Intraperitoneal ، Testis