Evaluation of Mir-۱۲۹۰ as a Possible Diagnostic Factor in the Serum of Oral Squamous Cell Carcinoma Patients with Qualitative Real-Time Polymerase Chain Reaction

Background & Objective: This study aimed to determine the incidence of microRNA (miRNA; miR-۱۲۹۰) in the serum of oral squamous cell carcinoma (OSCC) patients compared to a control group using the qualitative real-time polymerase chain reaction (PCR) method.Methods: Blood serum samples were obtained from patients diagnosed with OSCC and confirmed through biopsy. The samples were collected from patients referred to the Mashhad Dental Faculty and Ghaem Hospital. The OSCC group consisted of ۱۷ patients, while the healthy group included ۱۵ individuals. RNA was extracted from the patient samples, and samples with an A۲۶۰/۲۸۰ ratio between ۱.۸ and ۲.۰ (indicating acceptable RNA quality) were immediately converted into complementary DNA (cDNA) using albumin and cDNA reference genes. The SYBR green real-time reverse transcriptase PCR method was used to measure the presence of miR-۱۲۹۰ in the blood samples.Results: A total of ۳۲ patients were examined in this study, including ۱۷ women (۵۳.۱%) and ۱۵ men (۴۶.۹%). The mean age was ۴۶.۷ years in the healthy group and ۵۴.۶ years in the SCC group, indicating a significant difference (P<۰.۰۵). The expression level of the miR-۱۲۹۰ gene was higher in patients with SCC compared to the healthy group (P=۰.۰۰۰). While the expression level of miR-۱۲۹۰ was higher in grade ۳ and advanced stage than in grades ۲ and ۱ and early stage, the differences were not statistically significant (P=۰.۱۷۳ and P=۰.۵۶۴ for grade and stage, respectively).Conclusion: The expression level of miR-۱۲۹۰ may increase in SCC patients compared to healthy individuals, making it a potential circulating biomarker.  Further investigations for diagnostic utility would be warranted.

کلیدواژه ها:

biomarker ، miR-۱۲۹۰ ، Oral Squamous Cell Carcinoma ، Quantitative real-time polymerase chain reaction