Enriching potatoes with selenium is important considering the role that this plant has in the agricultural economy, feeding people and then improving the health of society. A greenhouse investigation was performed to examine the influence of selenium applied on the quality and quantity of potatoes at the Nutrition Research Center, Department of Food Hygiene and Quality Control, Shiraz University of Medical Sciences during ۲۰۲۱ using a randomized complete block design with four replications. Experimental treatments included control, foliar application of ۱۵ gr/ha sodium selenite, foliar application of ۳۰ gr/ha sodium selenite, soil applied of ۳ kg/ha sodium selenite, foliar application of ۱۵ gr/ha sodium selenite + soil applied of ۳ kg/ha sodium selenite, as well as foliar application of ۳۰ gr/ha sodium selenite + soil applied of ۳ kg/ha sodium selenite. The results showed that foliar application of different concentrations and soil application of ۳ kg/ha sodium selenite enhanced the selenium concentrations of potatoes; however, the most positive effects of sodium selenite were when selenium foliar application was applied with its soil application. It was found that the isoleysine amino acid content was not affected by sodium selenite applied; in contrast, foliar application and soil application of sodium selenite increased the concentration of glycine amino acid. The amount of soluble sugar and starch in potato tubers compared to the control increased under treatment with sodium selenite; however, the positive effects of foliar application of sodium selenite were high when applied to the soil. Sodium selenite reduced the nitrate content in potato tubers compared to the non-use of sodium selenite; in contrast, sodium selenite applied increased the nitrate-reductase enzyme activity, and the positive effects of foliar application of sodium selenite were high when applied with its soil applied. As well as the application of sodium selenite, which enhanced the potato tuber yield, the number of tubers improved compared to the non-use of this treatment. In conclusion, the soil application and foliar application of sodium selenite have a positive effect on the quantity and quality of potatoes as well as enrichment of selenium in potatoes; however, the positive effects of foliar application of sodium selenite were high when applied with its soil applied.