Correlation of ۲,۴-DDT with CDH۱ Gene Promoter Methylation in Gastric Cancer
سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 102
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شناسه ملی سند علمی:
JR_JKMU-31-1_001
تاریخ نمایه سازی: 27 اسفند 1402
چکیده مقاله:
AbstractBackground: Methylation alterations of tumor suppressor gene promoters are essential aspects of epigenetic changes in gastric cancer (GC). Cadherin۱ (CDH۱) encodes a protein with essential roles in cell-cell adhesions. In this study association of organochlorine pesticides (OCPs) serum levels with the methylation profile of this gene was investigated in gastric cancer (GC), intestinal metaplasia (IM), and functional dyspepsia (FD) patients.Methods: GC (n=۳۴), IM (n=۸), and FD (n=۴۸) patient serums were analyzed for the determination of OCP levels by gas chromatography equipment. The methylation status of the CDH۱ gene promoter was examined by methylation-specific PCR (MSP) method. In order to confirm reduced protein expression of this gene in methylated samples, immunohistochemistry (IHC) was used.Results: Our findings revealed significant hypermethylation of CDH۱ gene promoter along with reduced expression in GC patients compared with IM and FD patients. Furthermore, there was significant association between CDH۱ promoter hypermethylation and ۲,۴-DDT (odds ratio [OR]:۱.۱۸۳;۹۵%CI:۱.۰۰۱-۱.۳۹۸, p=۰.۰۴۸) serum levels in GC patients.Conclusion: Our results could suggest the association of ۲,۴-DDT OCP levels in the GC patient serums with CDH۱ gene promoter hypermethylation. Additionally, this gene promoter methylation may play a role in the progression of pre-cancerous IM towards GC.
کلیدواژه ها:
نویسندگان
Mohammad reza ashrafi
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
Gholamreza Asadi Karam
Applied Cellular and Molecular Research Center, Kerman University of Medical Sciences, Kerman, Iran
sadif darvishmoghddam
Gastroenterology and Hepatology Research Center, Kerman University of Medical Sciences, Kerman, Iranersity of Sciences-Iran
moslem abolhassani
Department of Clinical Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
maryam Iranpour
Pathology Department, Kerman faculty of medical science
zahra sepehri
Dept. of Biochemistry & Medical Genetics University of Manitoba