Molecular characterization of potential allergenic molds (Aspergillus, Penicillium and Fusarium) in two buildings receiving the public in Abidjan – Côte d’Ivoire

Aspergillus, Penicillium and Fusarium species play an important role in environmental allergy. These molds are potential density in the indoor environment. This study aimed to characterize the strains Aspergillus, Penicillium and Fusarium using a molecular method. The beta-tubulin and elongation factor TEF- genes were utilized to differentiate the species. A total of ۴۰۵ environmental species (Aspergillus, Penicillium and Fusarium) were isolated, purified and initially identified by colony morphology. Subsequently, DNA was extracted and; PCR was performed from ۳۵۱ isolates. The results were then compared to morphological characteristics. Of the ۳۵۱ isolates tested, ۱۶۷ were Aspergillus, ۱۰۳ were Penicillium and ۸۱ were Fusarium. The beta-tubulin and elongation factor TEF genes were found to be the most suitable for differentiating these three genera among them; the beta-tubulin gene was used for molecular identification of Aspergillus, Penicillium and the elongation factor TEF gene for characterizing Fusarium species.Aspergillus, Penicillium and Fusarium species play an important role in environmental allergy. These molds are potential density in the indoor environment. This study aimed to characterize the strains Aspergillus, Penicillium and Fusarium using a molecular method. The beta-tubulin and elongation factor TEF- genes were utilized to differentiate the species. A total of ۴۰۵ environmental species (Aspergillus, Penicillium and Fusarium) were isolated, purified and initially identified by colony morphology. Subsequently, DNA was extracted and; PCR was performed from ۳۵۱ isolates. The results were then compared to morphological characteristics. Of the ۳۵۱ isolates tested, ۱۶۷ were Aspergillus, ۱۰۳ were Penicillium and ۸۱ were Fusarium. The beta-tubulin and elongation factor TEF genes were found to be the most suitable for differentiating these three genera among them; the beta-tubulin gene was used for molecular identification of Aspergillus, Penicillium and the elongation factor TEF gene for characterizing Fusarium species.

کلیدواژه ها:

Allergy ، Moulds ، Aspergillus ، Penicillium ، Fusarium ، PCR.