Preparation and evaluation of latex agglutination technique for diagnosis and seroepidemiological studies of visceral leishmaniasis

in this study which has been carried out from ۱۹۹۶ until ۱۹۹۷ soluble latex agglutination antigen was prepared from L, donovani,sudan(s-۱) by culturing in special media,harvesting,and later solubilizing by freezing and thawing.the antigen were futher prepared by high speed centrifugation.after measurement of the protein (lowry method) concentration of the antigen was set at ۲.۲ mg/ml,the antigen was coated in ۲ to ۱ ratio with latex beads (۰.۸ mm) and futher diluted ۱:۱۰۰.following preparation of latex agglutination antigen,۲۱۰ human plasma samples and ۵۷ dog plasma samples from Meshkin-Shahr district were tested by latex agglutination test(LAT),direct agglutination test (DAT) and indirect flourescence antibody test (IFAT).also ۹۲۴ plasma samples from children >۹ years old and ۱۵ dog plasma samples from Kordan village,savegbolagh district,licated in Karaj in west of Tehran were tested by LAT,DAT and Enzyme linked immunosorbent assay(ELISA).the results show that the sensitivity of the LAT in human samples was ۹۸% and in dog samples ۹۶%.concordance between LAT and DAT in human samples is ۸۰% and in dog samples is ۸۹.۴%.

کلیدواژه ها:

latex agglutination ، visceral leishmaniasis ، Diagnosis ، Seroepidemiology