RNA-Binding Proteins as Key Regulators in Diabetic Nephropathy: A BioinformaticsApproach

Introduction: Diabetic nephropathy (DN) is a significant complication of diabetes mellitus, contributing tosubstantial global morbidity and mortality. Multiple factors, including systemic diseases, infections,autoimmune disorders, and genetic predisposition, can contribute to its development. This bioinformatics studyspecifically investigates the intricate molecular mechanisms involved in DN, with a particular emphasis on therole of RNA-binding proteins (RBPs). Through genomic analysis, our objective is to unveil regulatory networksand pinpoint key RBPs associated with the pathogenesis of DN.Methods: We conducted gene analysis on the GSE۱۴۲۱۵۳ dataset to identify significantly altered expression.Subsequently, we employed the Enrichr database to determine the ontology of these genes. Utilizing theCytohubba plugin, we identified the top genes. Finally, we investigated the interaction between RBP-mRNAusing RNAInter .Results: We analyzed the GSE۱۴۲۱۵۳ dataset, applying a significance threshold with a p-adjusted value < ۰.۰۵and |Log FC| > ۱ for gene selection, resulting in the identification of ۸۰ genes. Following this, we examined theprotein-protein interaction (PPI) STRING database with an average confidence level of ۰.۴. Subsequently, theMCC algorithm in Cytoscape was employed to identify ۱۰ gene hubs. Finally, using the RNAInter database toinvestigate RBP-mRNA interaction through weak experimental evidence method with a score of ۰.۲۵ to ۱, weidentified ۱۴ critical RBPs, including DHX۹, FMR۱, CAPRIN۱, ZC۳H۷B, HNRNPH۱, EIF۴G۲, DGCR۸,ACIN۱, DDX۵۴, EWSR۱, HNRNPA۱, UPF۱, ADAR and ELAVL۱ with a regulatory role in DN.Conclusion: This study enhances our comprehension of the molecular mechanisms in DN and underscores thecrucial role of RBPs as modulators. The discovery of novel RBP targets presents opportunities for developingtargeted therapeutic strategies, thereby advancing precision medicine in the context of diabetic complications

کلیدواژه ها:

Diabetic nephropathy ، RNA-binding proteins ، bioinformatics