Design a nanosensor based on silver nanocluster for detection of genetically modifiedorganisms
سال انتشار: 1402
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 38
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شناسه ملی سند علمی:
HUMS05_171
تاریخ نمایه سازی: 16 اسفند 1402
چکیده مقاله:
Introduction: Genetically modified organisms (GMOs) are produced by introducing transgene into the genomeof an organism to achieve a desirable trait.Methods: Nanoclusters (NCs) of noble metals, including Ag, Au, and Cu are molecule-like particles of ۲-nmaverage diameter. Here, for the first time, we have tuned physicochemical features of DNA-based silvernanocluster to obtain a novelready-to-use nanosensor for GMO detection. A DNA-silver nanocluster with dualmode emissions is devised, in which this unique modality has been exploited to develop a novel nanosensor fortransgenic DNA detection.Results: TEM and fluorescence analysis revealed the formation of Ag nanoclusters, which exhibit a weakemission at ۵۵۰ nm. Moreover, in the presence of the target sequence from the transgenic plant, the nanoclustersshowed a strong enhancement in emission. This property provided a suitable sensing platform which lacksunavoidable noises. The fluorescence emission of the nanoclusters exhibited a linear relation with the targetconcentration. However, the control DNA did not affect this ratio, which clearly confirmed the selectiveresponse of the designed nanosensor.Conclusion: Finally, the attractive features of the designed nanosensor may improve the current strategies forGMOs detection.
کلیدواژه ها:
نویسندگان
Seyedeh Nastaran Torab Jahromi
Bachelor's student of laboratory sciences - Student Research Committee, Jahrom University of Medical Sciences
Abazar Roostazade
Member of the Faculty of Jahrom University of Medical Sciences, Department of Advanced Medical Sciences &Technologies, School of Medicine, Jahrom University of Medical Sciences
Hassan Bardania
Assistant Professor of Cellular and Molecular Biology Research Center, Yasouj University of Medical Sciences
Mohsen Alipour
Member of the Faculty of Jahrom University of Medical Sciences, Department of Advanced Medical Sciences &Technologies, School of Medicine, Jahrom University of Medical Sciences