Study of Anti-Retroviral Effects of Salix Aegyptiaca L Herbal Extract on HIV-۱ in-vitro
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 84
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شناسه ملی سند علمی:
JR_IJMCM-4-1_010
تاریخ نمایه سازی: 16 اسفند 1402
چکیده مقاله:
Human immunodeficiency virus (HIV) infection is the major problem in the world. Nowadays, new anti-retroviral drugs are extracted from medicinal plants, make an important role to supersede synthetic drugs with different side effects. In this study, we studied the Salix aegyptiaca L extract, Iranian herb, as an anti-HIV drug with anti-retroviral effects. We used a single cycle replicable (SCR) HIV-۱ system by co-transfection of HEK ۲۹۳T with pmzNL۴-۳, psPAX۲ and pM۲G.۲. Cytotoxicity and cytopathic protection assay were performed by XTT method. Inhibition of p۲۴ Ag production level assay was done using quantitative enzyme linked immunosorbent assay (ELISA). Salix aegyptiaca L inhibited HIV-۱ induced CPE in HELA cells with SI value of ۲۲.۲. Finally, we use informatics analysis such as Gaussian, Hyper Chem , HF and B۳LYP methods and ۶-۳۱G, ۶-۳۱G** and ۶-۳۱۱G** basis sets. These results suggest that Salix aegyptiaca L has potent anti-HIV activity and may be a promising candidate for AIDS.
کلیدواژه ها:
Human immunodeficiency virus۱ ، Salix aegyptiaca L ، single cycle replicable virion (SCR) ، Gaussian ، HF ، Hyper Chem
نویسندگان
Yassaman Eftekhari
Department of Biochemistry, College of Basic Sciences, Tehran, Science and Research Branch, Islamic Azad University, Tehran, Iran
A Rustaiyan
Department of Chemistry, College of Basic Sciences, Tehran, Science and Research Branch, Islamic Azad University, Tehran, Iran.
M Monajjemi
Department of Chemistry, College of Basic Sciences, Tehran, Science and Research Branch, Islamic Azad University, Tehran, Iran.
R. A Khavari-nejad
Department of Biology, College of Basic Sciences, Tehran, Science and Research Branch, Islamic Azad University, Tehran, Iran