Isolation and Detection of Virulence factors of Salmonella typhimurium and Salmonella enteritidis
سال انتشار: 1391
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 81
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شناسه ملی سند علمی:
JR_IJMCM-2-2_001
تاریخ نمایه سازی: 16 اسفند 1402
چکیده مقاله:
Plasmids play an important role in the virulence and antibiotic resistance of Salmonella. Salmonella carry plasmids having different size and number. Plasmid profile analysis of single isolates of Salmonella typhimurium and Salmonella enteritidis revealed the presence of ۴- plasmid profiles. Salmonella serovars produce several Type III secretions including Sop-E and Sop –B. Soap E is detected by Dot-ELISA. Dot-ELISA is one of the solid phase immuneassay used to detect either antigens or antibody. Sop-B (Salmonella outer protein-B) is detected by multiplex PCR method using PCR primers Sop P۱ and SOP- M۱۳. It is concluded that the technique used in this study is more sensitive and identification and determination of various molecular characteristics of two species enhances the knowledge towards the vaccine production.
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نویسندگان
Nazir wani
Department of Microbiology, M.G.R. College, Dr M.G.R. Nagar Hosur. Periyar university, Salem. Tamil Nadu, India.
Rouf Ali
Department of Microbiology, M.G.R. College, Dr M.G.R. Nagar Hosur. Periyar university, Salem. Tamil Nadu, India.
Siva Shanmugam
Department of Microbiology, M.G.R. College, Dr M.G.R. Nagar Hosur. Periyar university, Salem. Tamil Nadu, India.