Pattern of macrolide resistance in Clostridium perfringens isolates from small ruminants in Kerman, Iran

BACKGROUND AND OBJECTIVESClostridium perfringens (C. perfringens) is an anaerobic spore-forming Gram-positive bacteria, ranking amongst the most important pathogens living in the intestinal tracts of both humans and animals. Its pathogenicity is largely attributed to produce several potent toxin and several extracellular enzymes which can cause histotoxic infections, food poisoning and a spectrum of intestinal diseases like enteritis and enterotoxaemia. Enterotoxaemia is a pathological condition characterized by the absorption of toxins produced within the intestinal tract, which ultimately leads to deleterious effects on several internal organs, including the brain, kidneys, lungs, and heart. It is one of the most frequently reported diseases of sheep and goats all over the world. In different countries, the prevalence rates of the enterotoxaemia ranges from ۲۴.۱۳ to ۱۰۰% in small ruminants. Antimicrobial agents are used for control and treatment of this disease and sometimes clinical outbreaks do not respond well to certain treatments. The objective of this study was to determine the resistance of C. perfringens isolates to macrolide antibiotic class.MATERIALS AND METHODSThis study was performed on a total of ۲۷۳ C. perfringens isolates, which were previously recovered from fecal samples of the diseased small ruminants in Kerman province, Iran. They were evaluated for the antimicrobial resistancy against tylosin and erythromycin (macrolide class) antibiotics by Kirby-Bauer disk diffusion method.RESULTS AND DISCUSSIONThe findings of our study demonstrated the high antibiotic resistant rate of C. perfringens isolates in small ruminants. The highest resistancy were observed to tylosin (۵۳.۱۱%), and erythromycin (۲۳.۴۴%) antibiotics, respectively.CONCLUSIONTo the best of our knowledge, this is the first study regarding the antimicrobial susceptibility of C. perfringens in small ruminants in Kerman province of Iran. This pattern of antibiotic resistance in C. perfringens, potentially reflecting the farm usage of these agents. Tight restriction of unnecessary antibiotic uses is necessary for some clostridial diseases

کلیدواژه ها:

Clostridium perfringens ، Macrolide ، Tylosin ، Erythromycin