Identification of some specific genes in Campylobacter jejuni associated with the response to acidic pH by using bioinformatics analysis

BACKGROUND AND OBJECTIVESCampylobacter jejuni is one of the leading causes of bacterial gastroenteritis and the most frequent cause of campylobacteriosis in the globe. This bacterium can have negative consequences and diseases like sepsis, meningitis, urinary tract infections, hemolytic uremic syndrome in people, and ailments in birds anywhere in the world. Due to the rapid rise of multidrug-resistant isolates, C. jejuni is categorized as a member of the pathogenic group. The flesh of birds and other animals is the most popular means of transmission to humans. It can be very helpful to understand how to regulate the diseases this bacterium causes by looking at how its genes are expressed under stress situations like an acidic pH. This was accomplished in this study using bioinformatics methods.MATERIALS AND METHODSGEO۲R web tools and R software are used to locate, extract, and analyze the microarray data with the accession ID GSE۹۹۳۷. Using the criteria P<۰.۰۵ and LogFC>|۱|, the genes with the greatest differential expression were found. After isolating the expression of the associated genes, the protein network for the genes that had increased expression was predicted by the STRING database and visualized using the Cytoscape program.RESULTS AND DISCUSSION۱۶۵۳ genes were obtained as DEGs (۸۸۹ upregulate, ۷۶۴ downregulate). Our analysis revealed ۵ hub genes, namely, secy(Sec export pathway or Sec-dependent pathway), cj۰۴۱۴(gluconate metabolism), acnb(aconitate hydratase B), cj۰۱۴۵(Twin-Arginine Translocation), ribd(riboflavin synthesis). Furthermore, the results of the KEGG pathway analysis revealed that these genes C. jejuni bacteria play a major role in pathogenesis.CONCLUSIONOverall, in this study we identified differentially expressed genes in C. jejuni in response to acidic pH through bioinformatics analysis of gene expression data. Identifying these genes provides valuable insights into the molecular pathways that C. jejuni employs to maintain itsviability, respond to environmental stressors and cause infections and aid in the identify development of more effective control measures for combating infections and designing preventive strategies.

کلیدواژه ها:

Campylobacter jejuni ، Acidic pH ، Pathogenesis ، Gene expression analysis ، bioinformatics.