Isolation and identification of grapevine endophytic bacteria and their extracted lipopeptides with antagonistic potential against Fomitiporia mediterranea

BACKGROUND AND ABJECTIVEThis study aimed to isolate and identify grapevine endophytic bacteria with the ability to produce lipopeptides for antagonistic activity against Fomitiporia mediterranea.MATERIALS AND METHODSIn this study, a total of ۶۲ bacteria were isolated from grapevine orchards of ۱۲ geographical regions located in Zanjan province. All of the isolates were evaluated in biochemical tests. Six selected strains were identified by nucleotide sequencing of the ۱۶S rRNA gene. The antifungal effect of isolated strains on four fungal species i.e. Chaetomium globosum, Cytospora chrysosperma, Fusarium sp., and Fomitiporia mediterranea was done by dual culture method. Also, the methanolic lipopeptide extract of strains extracted and the antagonistic activity of their on Fomitiporia mediterranea was investigated.RESULTS AND DISCUSSIONThe selected strains belonged to known genera including Bacillus, Pantoea, Serratia, and Variovorax. In the investigation of the antagonistic activity, ۱۱ isolates showed antifungal properties that two isolates of Serratia plymuthica and Variovorax sp. showed the most growth inhibitory zones. In addition, the methanolic lipopeptide extract of Serratia plymuthica isolate had the highest percentage of inhibition against Fomitiporia mediterranea.CONCLUSIONDue to the importance of endophytic bacteria and the production of bioactive lipopeptides by them, their isolation and identification from different parts of the country seem necessary, as a result, these bacteria and their lipopeptides extracts can be used in the biological control of plant diseases and can be replaced by chemical control.

کلیدواژه ها:

Endophytic bacteria ، Methanolic lipopeptide ، Antifungal activity ، Grapevine