Antibacterial and Antibiofilm activity of Diclofenac sodium in combination with Common Antibiotics against multidrug-resistant Pseudomonas aeruginosa

BACKGROUND AND OBJECTIVESThe increasing prevalence of multidrug-resistant (MDR) bacteria is a threat to public health, and the treatment of infections caused by them is associated with serious challenges. However, the combined use of antibiotics and non-antibiotic drugs could be beneficial in the treatment of MDR bacterial infection.MATERIALS AND METHODSIn this study, a total of ۱۰ P. aeruginosa clinical isolates were investigated. Kirby Bauer Disk diffusion method was used to determine the antibiotic susceptibility of the isolates. The minimal inhibitory concentrations (MICs), minimal biofilm inhibitory concentrations (MBICs), and minimum biofilm eradication concentrations (MBECs) of ciprofloxacin (CIP), meropenem (MEM), cefepime (FEP) and gentamicin (GEN) with/without sub-MICs of diclofenac sodium (DIC; ۵۰۰, ۱۰۰۰, ۲۰۰۰ μg/mL) for the clinical isolates were determined by the microbroth dilution and microtiter assay methods. the beginning of theRESULTS AND DISSCUSIONDIC significantly decreased the levels of MIC to FEP, MEM, and GEN in ۲۰۰۰ μg/mL, also the level of MBICs to FEP, GEN, CIP, and MEM among P. aeruginosa isolates significantly reduced in the presence of ۲۰۰۰ μg/mL of DIC. In this study, no significant changes were observed in the MBC level in the presence of diclofenac.CONCLUSIONOur study suggests that DIC could increase the efficacy of antibiotics against clinical isolates of P. aeruginosa and probably interferes with initial stages in biofilm formation although more investigation is needed.

کلیدواژه ها:

Antibacterial ، Antibiofilm ، Diclofenac sodium