Prevalence of Extended-Spectrum β-Lactamase in Pseudomonas aeruginosa Isolated from distinct Clinical specimen

BACKGROUND AND OBJECTIVESPseudomonas aeruginosa is a one of the most accepted causes of nosocomial infections. Resistance of P. aeruginosa to β-lactam antibiotics may be the result of acquired resistance through mutation and over production of various antibiotic inactivating enzymes. This probe aimed to rule the prevalence of extended-spectrum β-lactamases (ESBL) production as well as the presence of their related genes P. aeruginosa isolated from patients,MATERIALS AND METHODSThe antimicrobial susceptibility of ۲۰ P. aeruginosa isolates from patients was examined against ۱۳ antibiotics by the disc diffusion method. β-lactam/inhibitor (BLI) combined disks including ceftazidime/clavulanic acid and cefepime/clavulanic acid were considered for isolates that revealed resistance phenotype to cefepime and ceftazidime to detect ESBLs genes, multiplex PCR was done.RESULTS AND DISCUSSIONImipenem showed The highest resistance ۱۰۰% ,while the highest sensitivity to the antibiotic was Gentamycin (۵۵%) was observed.ESBL were observed in ۸(%۵۳). Overall, ۱۵ isolates (۷۵%) harbored bla AmpC (ICUs wards) , ۱ (۵%) had bla TEM and ۱ (۵%) carried bla PER genes. harbored bla AmpC , had bla PER ۱ (۵%) gene and bla SHV and bla OXA genes were not detected .CONCLUSIONA high prevalence of multiple β-lactamase production was observed among the AmpC producers (۷۵%). that the highest prevalence was observed in the intensive care unit and this conclusion emphasizes the correct implementation and monitoring of relevant protocols.

کلیدواژه ها:

Beta-Lactamase ، Pseudomonas aeruginosa ، blaESBL