Identification of the role of HPV in HNSCC tumors and margin healthy tissues and its association with demographic and clinicopathologic characteristics

BACKGROUND AND OBJECTIVESHuman papillomavirus (HPV) is a DNA virus in the papillomavirus family that is transmitted primarily through sexual contact. More than ۲۰۰ different genotypes of HPV have been identified, and some of these genotypes are specifically implicated in the occurrence of head and neck cancer. As HPV is known to be an important etiological factor in the development of head and neck squamous cell carcinoma (HNSCC). The aim of this study was to investigate the presence of HPV in both HNSCC tumor tissues and adjacent margin healthy tissues and to explore its association with demographic and clinicopathological characteristics.MATERIALS AND METHODSThis study was performed with a total of ۶۲ patients diagnosed with HNSCC. Tumor tissues and corresponding margin healthy tissues were collected from each patient. HPV detection was performed by polymerase chain reaction (PCR) for MY۰۹ and MY۱۱ genes. Demographic and clinicopathological data, including age, gender, smoking status, alcohol consumption, and tumor stage, were obtained from medical records.RESULTS AND DISCUSSIONThe results showed that HPV was detected in ۱۲.۹۰% of HNSCC tumor tissues and ۴.۸۳% of margin healthy tissues, which was significant (P=۰.۰۱). In the relationship between HPV status and demographic and clinical pathology characteristics, it was found that there was a significant association between HPV detection in tumor tissues and lymph node involvement (P=۰.۰۳) and histological grade ((P=۰.۰۰۳). The presence of positive HPV in tumor tissues showed a statistically significant association with older age (p < ۰.۰۵) and advanced tumor stage (p < ۰.۰۰۱). However, there was no significant association between HPV detection in tumor tissues and smoking, alcohol, and opium consumption.CONCLUSIONThis study demonstrates the presence of HPV in both HNSCC tumor tissues and adjacent margin healthy tissues. The higher prevalence of HPV in HNSCC tumor tissue and its relationship with demographic and clinical pathology characteristics demonstrates the importance of HPV testing in the diagnosis and management of HNSCC. These findings contribute to a better understanding of the role of HPV in HNSCC and may have implications for personalized treatment strategies in the future.

کلیدواژه ها:

Grade ، head and neck squamous cell carcinoma ، human papillomavirus ، stage