Investigation of anti-bacterial and anti-cancer effects of curcumin ferromagnetic nanocomposite on Acinetobacter baumannii isolates and AGS cell line

BACKGROUND AND OBJECTIVESCurcumin, a flavonoid from the rhizome of Curcuma longa, has anti-bacterial and anti-cancer activities. This study aimed to design and synthesize a magnetic nanocarrier to deliver curcumin to bacterial and cancer cells.MATERIALS AND METHODSThe physicochemical, anti-bacterial, and anti-cancer properties of curcumin ferromagnetic nanocomposite were measured. In addition, clinical isolates of Acinetobacter baumannii, gathered from medical and educational facilities in Tehran, Iran, were treated with ciprofloxacin (sub-MICs) alone and/or in combination with curcumin nanocomposites. The cytotoxicity effects of curcumin were studied on AGS cells.RESULTS AND DISCUSSIONThe physicochemical analyses have confirmed the synthesis of the proposed structure for curcumin ferromagnetic nanocomposite. Additional measurements have also been taken to determine the particle size and paramagnetic properties. The anti-bacterial property of curcumin in Fe۳O۴@SP particles decreased the expression of efflux pump and biofilm formation related genes and increased the porin genes in combination with ciprofloxacin in A. baumannii. MTT assay analysis showed that curcumin ferromagnetic nanocomposite inhibited cell proliferation in a dose‐ and time-dependent manner and had anti- invasive and anti-migrative effects on AGS cells.CONCLUSIONOur results suggested curcumin in ferromagnetic nanocomposite can used as anti-bacterial and anti-cancer agent.

کلیدواژه ها:

Acinetobacter baumannii ، Biofilm ، Curcumin ، Fe۳O۴ nanocarriers ، AGS cells